The impact of testicular and accessory sex gland function on sperm chromatin integrity as assessed by the sperm chromatin structure assay (SCSA)

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The impact of testicular and accessory sex gland function on sperm chromatin integrity as assessed by the sperm chromatin structure assay (SCSA).

BACKGROUND The sperm chromatin structure assay (SCSA) provides an objective assessment of sperm chromatin integrity, which is essential for normal sperm function. SCSA is valuable as a fertility marker in epidemiological studies and in the clinical situation. Little is known about the impact of testicular and post-testicular function on SCSA parameters. METHODS Ejaculates from 278 military co...

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Studies over the past two decades have clearly shown that reproductive toxicants cause sperm DNA fragmentation. This DNA fragmentation can usually be detected prior to observing alterations of metaphase chromosomes in embryos. Thus, Sperm Chromatin Structure Assay (SCSA)-detected DNA damage is viewed as the molecular precursor to later gross chromosome damage observed under the light microscope...

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The sperm chromatin structure assay (SCSA) as prognostic factor in IVF/ICSI program.

In this study 60 couples undergoing intracytoplasmic sperm injection (ICSI) procedures were enrolled. All men were classified into two groups regarding to the DNA fractionation index (DFI) threshold value: group I < 15% and group II =or> 15%. In group I, median DFI was 4%, normal pre-implantation embryo development was observed and eleven pregnancies were achieved. In group II, median DFI was 2...

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Sperm Chromatin Structure Assay (SCSA®): The Clinical Utility of Measuring Sperm DNA Damage and its Potential Improvement with Supplemental Antioxidants

The Sperm Chromatin Structure Assay (SCSA®) is the pioneering sperm DNA fragmentation assay that precisely measures the percent of sperm in a semen sample that have Sperm DNA Damage (SDD), a negative factor for successful pregnancy. The SCSA® is a rapid, dual parameter, computer driven assay with diligent unbiased flow cytometer measurements on five to ten thousand sperm per sample providing ri...

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Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility.

Diagnosis of male infertility has mainly been based on the World Health Organization (WHO) manual-based semen parameter's concentration, motility and morphology. It has, however, become apparent that none of these parameters are reliable markers for evaluation of the fertility potential of a couple. A search for better markers has led to an increased focus on sperm chromatin integrity testing i...

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ژورنال

عنوان ژورنال: Human Reproduction

سال: 2002

ISSN: 1460-2350

DOI: 10.1093/humrep/17.12.3162